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91.
Laddawan Krongpong Kunihiko Futami Takayuki Katagiri Makoto Endo Masashi Maita 《Fisheries Science》2008,74(5):1055-1061
Furazolidone, an antibacterial drug that was once widely used in the livestock industry and aquaculture, is now prohibited
in numerous countries. It is difficult to detect residual furazolidone because it is readily metabolized in animal tissues
but, by using and liquid chromatography coupled with tandem mass spectrometry, its metabolite, 3-amino-2-oxazolidinone (AOZ)
can be detected. Here we describe the validity of an enzyme-linked immunosorbent assay (ELISA) kit to detect AOZ in Japanese
eel Anguilla japonica tissue. ELISA is capable of detecting AOZ at 1.0 μg/kg in an eel sample with excellent accuracy and precision. Our results
show that ELISA is suitable for regulatory purposes and for studying the fate of AOZ residues in eel treated with furazolidone.
To measure the persistence of AOZ in eel tissues, eels (1.4–6.5g) were immersed in tanks containing 2 and 10 mg furazolidone/L
for 3 h, and then maintained in a tank supplying well water for the next 160 days. The half-lives of AOZ, calculated from
the linear terminal part of the excretion curve, were 25.0 days in muscle and 21.6 days in liver from fish exposed to 2 mg/L
furazolidone. In the eels treated with 10 mg/L furazolidone, by contrast, high levels of AOZ were detected in liver and muscle,
but the half-lives of AOZ were similar to those in fish treated with 2 mg/L furazolidone. The half-lives of AOZ in eel tissues
were prolonged by the condition of low water temperature. 相似文献
92.
93.
G.M. Bhopale S.R. Naik G.G. Bhave S.S. Naik A. Gogate 《Comparative immunology, microbiology and infectious diseases》1997,20(4):309-314
An enzyme linked immunosorbent assay (ELISA) using penicillinase was developed in the form of diagnostic kits (Toxokit-G and Toxokit-M) for the detection of IgG and IgM antibodies to Toxoplasma gondii. The performance of both the kits was compared with commercially available diagnostic kits, i.e. Enzygnost-Toxoplasmosis/IgG (Behring Co., Germany), TOXOTEK-G (Flow Lab., U.K.) and Toxoplasma IgM Microassay (Diamedix Corp., U.S.A.) by testing toxoplasma-suspected human serum samples. The results indicate a good reliability between these diagnostic kits. Toxokit-G has 86.66 and 96.05% sensitivity and specificity respectively. The main advantage of Toxokit-G is that the end result can be assessed visually without using sophisticated instruments. Toxokit-M has 100% sensitivity and specificity and test results were not affected by the presence of antitoxoplasma IgG antibodies, rheumatoid factor or antinuclear antibodies. 相似文献
94.
C. Secchi L. Oldani C. Peruccio M. Guttinger A. Berrini C. Caldora G. Poli 《Comparative immunology, microbiology and infectious diseases》1985,8(3-4):297-303
Canine retinal S antigen has been purified to study the retinal progressive atrophy of the dog. The purified antigen will be used to detect, by the ELISA technique, specific autoantibody in dogs with ocular diseases. 相似文献
95.
Besma Sghaier Walid Kriaa Mouna Bahloul Jesús V. Jorrín Novo Noureddine Drira 《Scientia Horticulturae》2009
Abscisic acid (ABA), arginine and sucrose were evaluated for their effects on the morphology, germination rates and protein content of date palm somatic embryos (SE). Different concentrations of these supplements in the culture medium were used. The comparative study of SE length and thickness between treated and untreated SE revealed no differences, except for ABA (20 μM), which increased thickness. A decrease of water content (WC) in favor of an increase in dry weight (DW) was observed in all treated SE, especially with sucrose (90 g l−1) and ABA (20 μM). Only ABA (20 and 4 μM) caused a proliferation rate of the cultures higher than those in the control. Although all the tested compounds increased protein content, ABA (20 μM) was more effective in protein enrichment than arginine and sucrose treatments. The SDS-PAGE protein profiles showed a significant difference between treated and untreated SE. A protein band of 22 kDa, identified as glutelin in a previous work, was accumulated after treatment with 20 μM ABA or 3 mM arginine. These findings may contribute to further understanding of the mechanisms involved in the accumulation of specific storage proteins in several plants. 相似文献
96.
采用ELISA方法、分光光度计法和组织学方法,系统研究了不同年龄小体鲟(Acipenser ruthenus)血清卵黄蛋白原(Vitellogenin Vg)、血清钙离子(Galcium Ca2+)含量与卵巢发育变化的关系.结果表明:血清Vg浓度、Ca2+浓度与卵巢的发育时期相关;在卵黄沉积期内,血清中Vg浓度与Ca2+浓度呈线性正相关(R2=0.926 4);卵黄生成前期和沉积初期,血清中Vg浓度和Ca2+含量较低;卵黄沉积期内,卵母细胞由沉积初期的0.56 mm 增大到卵黄沉积末期的2.0l mm,血清Vg质量浓度由110.65 μg/mL 上升到242.22 μg/mL,血清Ca2+浓度由2.34 mol/L增大到2.72 mol/L.对同年龄的雌、雄小体鲟血清Vg浓度和Ca2+浓度测定表明,根据血清中Vg和Ca2+含量差别,可以区分出沉积期小体鲟的性别. 相似文献
97.
建立了检测包虫病循环抗原的夹心酶联免疫吸附试验。对130份阳性血清(猪65,羊65)作了检测,总阳性率为79.2%,抗原最小检出量为50μg/L。 相似文献
98.
HANG Tianyu ZHAO Hongzhe SONG Qianjin ZHANG Jing JI Zhi WEN Yongjun GUAN Pingyuan 《中国畜牧兽医》2007,47(11):3641-3650
In this study,the genome of Brucella Rev.1 strain was used as a template to amplify the BAB gene sequence,clone it into the prokaryotic expression vector pET-30a(+),obtain the recombinant plasmid pET-30a-BAB,and perform prokaryotic expression on the recombinant plasmid.The indirect ELISA method was constructed by using the expression products detected by Western blotting.The results showed that the BAB gene was successfully cloned and expressed in this experiment,and the purified expression product was analyzed by SDS-PAGE.This study obtained a relatively pure recombinant BAB protein;Western blotting test showed that the expressed protein could react specifically with Brucella sheep positive serum and had good reactogenicity;Using the recombinant BAB protein as the coating antigen,an indirect ELISA method for detecting BAB antibodies was established and optimized.The best determined coating conditions were as follows BAB protein coating amount was 0.25 μg/mL,serum dilution was 1:400;blocking solution was 3% pig-derived gelatin;secondary antibody dilution was 1:6 000;color development time was 10 min.The established method was used to detect 40 clinical sheep serums,and the cut-off value was calculated to be 0.607.That was,when the serum tested had P/N ≥ 1.5 and D450 nm ≥ 0.607,it was judged as positive,when D450 nm ≤ 0.561,it was judged as negative,and when 0.607<D450 nm<0.561,it was judged as a suspect value,and retest was required.Compared with the Huhong plate test and the test tube agglutination test,the positive coincidence rate was 100%,the negative coincidence rate was 71.88%,and the total coincidence rate was 77.5%. 相似文献
99.
间接酶联免疫吸附试验检测猪生殖与呼吸系统综合征血清抗体的研究 总被引:2,自引:0,他引:2
本研究建立了检测猪生殖与呼吸系统综合征(PRRS)血清抗体的间接酶联免疫吸附试验(ELISA),其抗原包被浓度为2μg/ml,血清最适稀释度为1∶100。试验结果表明:ELISA的敏感性高于间接免疫荧光抗体试验(IFA),是一种切实可行的诊断方法。 相似文献
100.
应用改良阻断ELISA检测禽网状内皮组织增殖病血清抗体 总被引:3,自引:0,他引:3
应用禽网状内皮组织增殖病病毒纯化抗原和抗REV单克隆抗体建立了改良阻断ELISA用于鸡血清中REV抗体检测,并对北京地区鸡群中随机采样的36份血清样本进行了检测,阳性率为5.6%。与间接ELSIA的检测结果进行了统计学比较,两种方法的阳性率无显著差异。结果表明本试验所建立的改良阻断ELISA可以用于鸡群REV感染的血清学调查。 相似文献